PRODUCT CLASSIFICATION
产物分类Oxford Expression Technologies 重组蛋白
Oxford Expression Technologies公司介绍
Oxford Expression Technologies(英国)位于英国牛津,为全球制药和生物技术行业提供产物,服务和咨询服务。
Oxford Expression Technologies 简称(OET)专门从事重组蛋白表达,其核心技flashBACTM是一种基于杆状病毒的蛋白表达系统,能快速生产适用于小型和大型蛋白表达系统的多种重组病毒。
Oxford Expression Technologies简称(OET)产物还涉及昆虫和哺乳动物表达系统中的载体、转染试剂和培养基。
蛋白质印迹
该分析使用凝胶电泳分离的提取物中抗体与变性蛋白质的结合。它对于识别与其他蛋白质的脱靶结合非常有用。它还可以与二维分析相结合,二维分析在第一维通过电荷分离蛋白质,然后在第二维通过大小分离。这种更先进的方法可识别与预期蛋白质大小相同的非靶蛋白是否与抗体结合。
共聚焦显微镜
我们可以使用牛津布鲁克斯大学(Oxford Brookes University)全驳耻辞最虫颈补苍进的共聚焦显微镜设备之一。共聚焦显微镜是研究抗体在细胞内非变性状态下与其靶标相互作用的理想选择。任何所需的细胞培养物都可以用重组杆状病毒或阴性对照感染(昆虫)或转导(哺乳动物和禽)。然后用一抗处理细胞,然后用标记的二抗孵育,该二抗在适当波长的光下产生荧光。
酶联免疫吸附法
这是抗体最常见的应用之一,特别是在诊断试剂盒中。至关重要的是,使用的任何抗体都可以被信任以结合正确的蛋白质靶标。在此类测试中,经过验证的阳性对照至关重要。虽然可以使用来自表达重组蛋白的细胞的粗提取物,但最好使用纯化的材料。这也是提供阴性对照很重要的地方。我们可以模拟从这些样品中提取蛋白质,并证明重组蛋白来源中没有其他任何东西可以与靶抗体结合。
宿主细胞的选择
大多数重组蛋白表达系统局限于狭窄范围的宿主细胞。杆状病毒系统的一个主要优点是,它可用于在生产性病毒感染后在昆虫细胞中制造蛋白质,或通过转导在哺乳动物和禽类细胞培养物中制造蛋白质。由于蛋白质的翻译后修饰可能是抗体结合的关键因素,因此使用来自细胞的材料来测试这一点是值得的,因为这些材料与预期靶标密切相关。
Oxford Expression Technologies公司的热销产物:
产物名称 | 货号 | 规格 |
flashBAC | OET-100150 | 5 reactions |
flashBAC | OET-100151 | 24 reactions |
flashBAC | OET-100152 | 96 reactions |
flashBAC GOLD | OET-100200 | 3 reactions |
flashBAC GOLD | OET-100201 | 5 reactions |
flashBAC GOLD | OET-100202 | 24 reactions |
flashBAC GOLD | OET-100203 | 96 reactions |
flashBAC ULTRA | OET-100300 | 5 reactions |
flashBAC ULTRA | OET-100301 | 24 reactions |
flashBAC ULTRA | OET-100302 | 96 reactions |
flashBAC ULTRA | OET-100304 | 3 reactions |
flashBAC selection box 1 | OET-100400 | 3 x 3 reactions |
flashBAC selection box 2 | OET-100401 | 4 x 3 reactions |
flashBAC PRIME | OET-100500 | 5 reactions |
flashBAC PRIME | OET-100501 | 24 reactions |
baculoQUANT All-in-one virus extraction & titration kit | OET-100602 | 100 reactions |
titrePLUS flashBAC all-in-one | OET-100710 | 5 + 100 reactions |
titrePLUS flashBAC all-in-one | OET-100711 | 24 + 100 reactions |
titrePLUS flashBAC GOLD all-in-one | OET-100712 | 5 + 100 reactions |
titrePLUS flashBAC GOLD all-in-one | OET-100713 | 24 + 100 reactions |
titrePLUS flashBAC ULTRA all-in-one | OET-100714 | 5 + 100 reactions |
titrePLUS flashBAC ULTRA all-in-one | OET-100715 | 24 + 100 reactions |
titrePLUS flashBAC PRIME all-in-one | OET-100716 | 5 + 100 reactions |
titrePLUS flashBAC PRIME all-in-one | OET-100717 | 24 + 100 reactions |
BacPAK6 Linearised DNA | OET-101101 | 5 reactions |
BacPAK6 Linearised DNA | OET-101102 | 24 reactions |
BacPAK6 Linearised DNA | OET-101103 | 96 reactions |
BacPAK6 sec+ Linearised DNA | OET-101104 | 5 reactions |
BacPAK6 sec+ Linearised DNA | OET-101105 | 24 reactions |
BacPAK6 sec+ Linearised DNA | OET-101106 | 96 reaction kit |
pOET sequencing primers | OET-200100 | 2 x 100 ul |
pOET1 transfer plasmid | OET-200101 | 10 ug |
pOET1N_6xHis transfer plasmid | OET-2001011 | 10 ug |
pOET1C_6xHis transfer plasmid | OET-2001012 | 10 ug |
pOET2 transfer plasmid | OET-200103 | 10 ug |
pOET2N/C_6xHis transfer plasmid | OET-2001031 | 10 ug |
pOET2C_6xHis transfer plasmid | OET-2001032 | 10 ug |
pOET3 transfer plasmid | OET-200104 | 10 ug |
pOET4 transfer plasmid | OET-200105 | 10 ug |
pOET5 transfer plasmid | OET-200106 | 10 ug |
pOET6 BacMAM transfer plasmid | OET-200107 | 10 ug |
pOET1 Gateway® transfer plasmid | OET-200108 | 6 ug |
pOET6 BacMAM Gateway® transfer plasmid | OET-200109 | 6 ug |
pOET8.VE1 transfer plasmid | OET-200121 | 10 ug |
pOET8.VE2 transfer plasmid | OET-200122 | 10 ug |
pOET8.VE3 transfer plasmid | OET-200123 | 10 ug |
baculoFECTIN II | OET-300105 | 150 ul |
baculoFECTIN II | OET-300106 | 1ml |
baculoCOMPLETE protein expression kit | OET-400100 | 5 reactions |
baculoCOMPLETE protein expression kit + baculoQUANT all-in-one | OET-400101 | 5 + 100 reactions |